Multidimensional LC–MS/MS analysis of liverproteins in rat, mouse and human microsomaland S9 fractions

Golizeh, Makan; Schneider, Christina; Ohlund, Leanne et Sleno, Lekha (2015). « Multidimensional LC–MS/MS analysis of liverproteins in rat, mouse and human microsomaland S9 fractions ». EuPA Open Proteomics, 6, pp. 16-27.

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Résumé

Liver plays a key role in metabolism and detoxification, therefore analysis of its proteome is relevant for toxicology and drug discovery studies. To optimize for high proteome cov-erage, protein and peptide-level ion exchange fractionation were assessed using rat livermicrosomes and S9 fractions. 2D-(SCX-RP)-LC–MS/MS analysis with peptide fractionation was subsequently employed for rat, mouse and human samples, yielding between 1400 and 1939 identified proteins, 58% of which were shared between species, and with relatively high sequence coverage. This rich dataset is specifically interesting for the toxicology community, and could serve as an excellent source for targeted assay developmen.t

Type:	Article de revue scientifique
Mots-clés ou Sujets:	Multidimensional liquid chromatography, High resolution tandem mass spectrometry, Liver proteins, Microsomal fractions, S9 fractions, Cross-species analysis
Unité d'appartenance:	Faculté des sciences > Département de chimie
Déposé par:	Lekha Sleno
Date de dépôt:	11 août 2021 08:56
Dernière modification:	11 août 2021 08:56
Adresse URL :	http://archipel.uqam.ca/id/eprint/14461

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