Identification of In Vitro Metabolites of Synthetic Phenolic Antioxidants BHT, BHA, and TBHQ by LC-HRMS/MS

Ousji, Ons et Sleno, Lekha (2020). « Identification of In Vitro Metabolites of Synthetic Phenolic Antioxidants BHT, BHA, and TBHQ by LC-HRMS/MS ». Int. J. Mol. Sci., 21, pp. 9525-9537.

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Résumé

Butylated hydroxytoluene (BHT) and its analogs, butylated hydroxyanisole (BHA) and tert-butyl-hydroquinone (TBHQ), are widely used synthetic preservatives to inhibit lipid oxidation in the food, cosmetic and pharmaceutical industries. Despite their widespread use, little is known about their human exposure and related biotransformation products. The metabolism of these compounds was investigated using in vitro incubations with human and rat liver fractions. Liquid chromatography coupled to high-resolution tandem mass spectrometry was employed to detect and characterize stable and reactive species formed via oxidative metabolism, as well as phase II conjugates. Several oxidative metabolites have been detected, as well as glutathione, glucuronide, and sulfate conjugates, many of which were not previously reported. A combination of accurate mass measurements, MS/MS fragmentation behavior, and isotope-labeling studies were used to elucidate metabolite structures.

Type:	Article de revue scientifique
Mots-clés ou Sujets:	BHT; BHA; synthetic antioxidant; metabolites; in vitro incubations; liquid chromatography; high-resolution tandem mass spectrometry
Unité d'appartenance:	Faculté des sciences > Département de chimie
Déposé par:	Lekha Sleno
Date de dépôt:	11 août 2021 08:55
Dernière modification:	11 août 2021 08:55
Adresse URL :	http://archipel.uqam.ca/id/eprint/14459

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