Characterization of a novel transgenic mouse line expressing Cre recombinase under the control of the Cdx2 neural specific enhancer

Coutaud, Baptiste et Pilon, Nicolas (2013). « Characterization of a novel transgenic mouse line expressing Cre recombinase under the control of the Cdx2 neural specific enhancer ». Genesis, 51(11), pp. 777-784.

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Résumé

Several genetically modified mouse models have been generated in order to drive expression of the Cre recombinase in the neuroectoderm. However, none of them specifically targets the posterior neural plate during neurulation. To fill this gap, we have generated a new transgenic mouse line in which Cre expression is controlled by a neural specific enhancer (NSE) from the Caudal‐related homeobox 2 (Cdx2) locus. Analyses of Cre activity via breeding with R26R‐YFP reporter mice have indicated that the Cdx2NSE‐Cre mouse line allows for recombination of LoxP sites in most cells of the posterior neural plate as soon as from the head fold stage. Detailed examination of double‐transgenic embryos has revealed that this novel Cre‐driver line allows targeting the entire posterior neural tube with an anterior limit in the caudal hindbrain. Of note, the Cdx2NSE regulatory sequences direct Cre expression along the whole dorso‐ventral axis (including pre‐migratory neural crest cells) and, accordingly, YFP fluorescence has been also observed in multiple non‐cranial neural crest derivatives of double‐transgenic embryos. Therefore, we believe that the Cdx2NSE‐Cre mouse line represents an important novel genetic tool for the study of early events occurring in the caudal neuroectoderm during the formation of both the central and the peripheral nervous systems.

Type:	Article de revue scientifique
Informations complémentaires:	This is the peer reviewed version of the following article: Coutaud, B. and Pilon, N. (2013), Characterization of a novel transgenic mouse line expressing Cre recombinase under the control of the Cdx2 neural specific enhancer. Genesis, 51: 777-784, which has been published in final form at https://onlinelibrary.wiley.com/doi/full/10.1002/dvg.22421. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
Mots-clés ou Sujets:	Cre recombinase, Cdx2, neural specific enhancer, posterior neural plate, neural tube, neural crest cells
Unité d'appartenance:	Centres institutionnels > Centre de recherches biomédicales (BIOMED) Faculté des sciences > Département des sciences biologiques
Déposé par:	Jean-Jacques Rondeau
Date de dépôt:	18 janv. 2019 11:36
Dernière modification:	18 janv. 2019 11:36
Adresse URL :	http://archipel.uqam.ca/id/eprint/12099

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