Male-specific colon motility dysfunction in the TashT mouse line

Touré, Aboubacrine M.; Charrier, Baptiste et Pilon, Nicolas (2016). « Male-specific colon motility dysfunction in the TashT mouse line ». Neurogastroenterology & Motility, 28(10), pp. 1494-1507.

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Background In Hirschsprung disease (HSCR), the absence of myenteric neural ganglia in the distal bowel prevents motility and thereby causes functional intestinal obstruction. Although surgical resection of the aganglionic segment allows HSCR children to survive this condition, a number of patients still suffer from impaired motility despite having myenteric ganglia in their postoperative distal bowel. Such phenomenon is also observed in patients suffering from other enteric neuropathies and, in both cases, colonic dysmotility is believed to result from abnormalities of myenteric ganglia and/or associated interstitial cells of Cajal (ICC). To better understand this, we used a recently described HSCR mouse model called TashT. Methods Intestinal motility parameters were assessed and correlated with extent of aganglionosis and with neuronal density in ganglionated regions. The neural composition of the myenteric plexus and the status of ICC networks was also evaluated using immunofluorescence. Key Results TashTTg/Tg mice display a strong male bias in the severity of both colonic aganglionosis and hypoganglionosis, which are associated with male‐specific reduced colonic motility. TashTTg/Tg male mice also exhibit a specific increase in nNos+ neurons that is restricted to the most distal ganglionated regions. In contrast, Calretinin+ myenteric neurons, Sox10+ myenteric glial cells, and cKit+ ICC are not affected in TashTTg/Tg mice. Conclusions and Inferences Male‐specific impairment of colonic motility in TashTTg/Tg mice is associated with both severe hypoganglionosis and myenteric neuronal imbalance. Considering these parameters in the clinic might be important for the management of postoperative HSCR patients.

Type: Article de revue scientifique
Informations complémentaires: This is the peer reviewed version of the following article: Touré, A. M., Charrier, B. and Pilon, N. (2016), Male‐specific colon motility dysfunction in the TashT mouse line. Neurogastroenterol. Motil., 28: 1494-1507, which has been published in final form at This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
Mots-clés ou Sujets: chronic constipation, enteric nervous system, Hirschsprung disease, myenteric plexus
Unité d'appartenance: Centres institutionnels > Centre de recherches biomédicales (BIOMED)
Faculté des sciences > Département des sciences biologiques
Déposé par: Jean-Jacques Rondeau
Date de dépôt: 18 janv. 2019 11:45
Dernière modification: 18 janv. 2019 11:45
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