Caudal-related Homeobox (Cdx) Protein-dependent Integration of Canonical Wnt Signaling on Paired-box 3 (Pax3) Neural Crest Enhancer

Sanchez-Ferras, Oraly; Coutaud, Baptiste; Samani, Taraneh Djavanbakht; Tremblay, Isabelle; Souchkova, Ouliana et Pilon, Nicolas (2012). « Caudal-related Homeobox (Cdx) Protein-dependent Integration of Canonical Wnt Signaling on Paired-box 3 (Pax3) Neural Crest Enhancer ». Journal of Biological Chemistry, 287(20), pp. 16623-16635.

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Résumé

One of the earliest events in neural crest development takes place at the neural plate border and consists in the induction of Pax3 expression by posteriorizing Wnt·β-catenin signaling. The molecular mechanism of this regulation is not well understood, but several observations suggest a role for posteriorizing Cdx transcription factors (Cdx1/2/4) in this process. Cdx genes are known as integrators of posteriorizing signals from Wnt, retinoic acid, and FGF pathways. In this work, we report that Wnt-mediated regulation of murine Pax3 expression is indirect and involves Cdx proteins as intermediates. We show that Pax3 transcripts co-localize with Cdx proteins in the posterior neurectoderm and that neural Pax3 expression is reduced in Cdx1-null embryos. Using Wnt3a-treated P19 cells and neural crest-derived Neuro2a cells, we demonstrate that Pax3 expression is induced by the Wnt-Cdx pathway. Co-transfection analyses, electrophoretic mobility shift assays, chromatin immunoprecipitation, and transgenic studies further indicate that Cdx proteins operate via direct binding to an evolutionarily conserved neural crest enhancer of the Pax3 proximal promoter. Taken together, these results suggest a novel neural function for Cdx proteins within the gene regulatory network controlling neural crest development.

Type: Article de revue scientifique
Informations complémentaires: This research was originally published in the Journal of Biological Chemistry. Oraly Sanchez-Ferras, Baptiste Coutaud, Taraneh Djavanbakht Samani, Isabelle Tremblay, Ouliana Souchkova and Nicolas Pilon. Caudal-related Homeobox (Cdx) Protein-dependent Integration of Canonical Wnt Signaling on Paired-box 3 (Pax3) Neural Crest Enhancer. J. Biol. Chem. 2012; Vol. 287: pp. 16623–16635. © the American Society for Biochemistry and Molecular Biology
Mots-clés ou Sujets: Wnt Signaling Pathway, murine Pax3 expression, Cdx proteins, P19 cells, neural crest cells, Neuro2a cells
Unité d'appartenance: Centres institutionnels > Centre de recherches biomédicales (BIOMED)
Faculté des sciences > Département des sciences biologiques
Déposé par: Jean-Jacques Rondeau
Date de dépôt: 07 janv. 2019 15:36
Dernière modification: 07 janv. 2019 15:36
Adresse URL : http://archipel.uqam.ca/id/eprint/12039

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