Dhh‐expressing Schwann cell precursors contribute to skin and cochlear melanocytes, but not to vestibular melanocytes

Bonnamour, Grégoire; Soret, Rodolphe et Pilon, Nicolas (2020). « Dhh‐expressing Schwann cell precursors contribute to skin and cochlear melanocytes, but not to vestibular melanocytes ». Pigment Cell & Melanoma Research, pp. 1-7.

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Résumé

For a long time, melanocytes were believed to be exclusively derived from neural crest cells migrating from the neural tube toward the developing skin. This notion was then challenged by studies suggesting that melanocytes could also be made from neural crest‐derived Schwann cell precursors (SCPs) on peripheral nerves. A SCP origin was inferred from lineage tracing studies in mice using a Plp1 promoter‐controlled Cre driver transgene (Plp1‐CreERT2) and a fluorescent Rosa26 locus‐controlled Cre reporter allele (Rosa26-FloxSTOP‐YFP). However, doubts were raised in part because another SCP‐directed Cre driver controlled by the Dhh promoter (Dhh‐Cre) was apparently unable to label melanocytes when used with a non‐fluorescent Rosa26 locus‐controlled Cre reporter (Rosa26-FloxSTOP‐LacZ). Here, we report that the same Dhh‐Cre driver line can efficiently label melanocytes when used in a pure FVB/N background together with the fluorescent instead of the non‐fluorescent Rosa26 locus‐controlled Cre reporter. Our data further suggest that the vast majority of skin melanocytes are SCP‐derived. Interestingly, we also discovered that SCPs contribute inner ear melanocytes in a region‐specific manner, extensively contributing to the cochlea but not to the vestibule.

Type: Article de revue scientifique
Informations complémentaires: This is the peer reviewed version of the following article: Bonnamour, G, Soret, R, Pilon, N. Dhh‐expressing Schwann cell precursors contribute to skin and cochlear melanocytes, but not to vestibular melanocytes. Pigment Cell Melanoma Res. 2020; 00: 1– 7. https://doi.org/10.1111/pcmr.12938 , which has been published in final form at https://onlinelibrary.wiley.com/doi/full/10.1111/pcmr.12938. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
Mots-clés ou Sujets: Cochlea, Cre/LoxP, Dhh, genetic cell lineage tracing, inner ear melanocytes, mouse models, Plp1, Schwann cell precursors, skin melanocytes, vestibule
Unité d'appartenance: Faculté des sciences > Département des sciences biologiques
Déposé par: Nicolas Pilon
Date de dépôt: 01 déc. 2020 11:33
Dernière modification: 21 oct. 2021 23:10
Adresse URL : http://archipel.uqam.ca/id/eprint/13778

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